Translational_Unit

Part:BBa_K993002:Design

Designed by: İBRAHİM YASİR ORHAN   Group: iGEM13_AUC_TURKEY   (2013-06-14)


IbpB RNA Thermometer with RFP


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 763
    Illegal AgeI site found at 875
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

Note that RNA thermometer actually works as an RBS sequence. Therefore, this part does not include an additional ribosome binding site. Also, this part does not contain an terminator sequence, but a simple stop codon. The RFP should be translated continuously.


Source

Escherichia coli, NCBI GeneBank Other elements are obtained from Registry.

References